Welcome!

The Cancer Discovery Hub (CDH) is a one-stop multi-omics molecular diagnostic core facility housed at the National Cancer Centre Singapore. We specialise in state-of-the-art research-grade technologies and provide expert support to investigators on all cancer related projects.

Lab Experiments
 

RECENT UPDATES

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SERVICES AVAILABLE

 
Web Consultation

Project consultation

Discussion on study feasibility, experimental design, manpower requirements, project costs, service needs and collaboration opportunities.

Sample extraction

Sample extraction

Provides a range of genomic material (e.g. DNA/RNA) extraction services from fresh or FFPE tissue, as well as blood-based analytes, including stringent QC  before downstream NGS applications.

NGS library preparation

Range of NGS services from custom target capture and amplicon sequencing. Provides direct liaison with established third-party NGS vendors. Custom epigenetics assays e.g. ChIP-seq and DNA methylation sequencing, are also available on case-by-case basis.

Blood Sample

NanoString nCounter system

The NanoString nCounter MAX system utilizes a novel digital colour-coded barcode technology that is based on direct multiplexed measurement of gene expression and offers high levels of precision and sensitivity (<1 copy per cell).

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Silicon Biosystems DEPArray system

Unique automated system for trapping, manipulation, and recovery of selected rare individual cells for downstream analysis of live or fixed cells, including next generation sequencing applications.

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10X Genomics Chromium

High throughput single cell transcriptomic analysis via 3’ or 5' end counting determines gene expression and characterizes cells of a heterogeneous population. In addition to expression profiling, the 5' assay enables immune profiling by enriching barcoded cDNA for V(D)J sequences of T or B cells.

10X Genomics Visium

State-of-the-art technology that is able to combine spatially-resolved whole transcriptome analysis with morphological context, complementing single cell gene expression information.

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Bioinformatics services

Range of services including basic QC metrics, variant calling, mutation signature analysis, metagenomics, gene expression and pathway analyses.

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Embryonic Stem Cells

Tumor model creation

Range of customized tumor model creation services including patient-derived xenografts, primary cell-lines, organoids (in development). Contact us for more details.

DNA Chips

Functional assays

Western blot, polymerase chain reaction, immunofluorescence, immunohistochemistry, laser capture microdissection, cell-based assays etc. can be provided on a case-by-case basis. Contact us for more details.

FEATURED PUBLICATIONS

Scientific publications supported by the Cancer Discovery Hub

HER2 expression, copy number variation and survival outcomes in HER2-low non-metastatic breast cancer: an international multicentre cohort study and TCGA-METABRIC analysis
BMC Medicine. 2022

Mar, 2022

HER2-low breast cancer (BC) is currently an area of active interest. This study evaluated the impact of low expression of HER2 on survival outcomes in HER2-negative non-metastatic breast cancer (BC).

Patients with HER2-negative non-metastatic BC from 6 centres within the Asian Breast Cancer Cooperative Group (ABCCG) (n = 28,280) were analysed. Results: ABCCG cohort median follow-up was 6.6 years; there were 12,260 (43.4%) HER2-low BC and 16,020 (56.6%) HER2-zero BC. The outcomes were better in HER2-low BC than in HER2-zero BC (RFS: centre-adjusted hazard ratio (HR) 0.88, 95% CI 0.82-0.93, P < 0.001; OS: centre-adjusted HR 0.82, 95% CI 0.76-0.89, P < 0.001). On multivariable analysis, HER2-low status was prognostic (RFS: HR 0.90, 95% CI 0.85-0.96, P = 0.002; OS: HR 0.86, 95% CI 0.79-0.93, P < 0.001). These differences remained significant in hormone receptor-positive tumours and for OS in hormone receptor-negative tumours. Superior outcomes were observed for HER2 IHC1+ BC versus HER2-zero BC (RFS: HR 0.89, 95% CI 0.83-0.96, P = 0.001; OS: HR 0.85, 95% CI 0.78-0.93, P = 0.001). Conclusions: HER2-low BC had a superior prognosis compared to HER2-zero BC in the non-metastatic setting, though absolute differences were modest and driven by HER2 IHC 1+ BC. ERBB2 CNV merits further investigation in HER2-negative BC.

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